Overview

This template as well as many protocols on this site are modified from the McClean lab’s OpenWetLab.

This is a template for how to write a new protocol in the GitHub repo for our lab. Anyone affiliated with our lab can contribute to this site. Just ask Bin to add you to the lab GitHub group, and learn how to use either the GitHub website, or preferably, the command line interface, to add new files. In your real protocol, a description of what the protocol is and when to use it would go here.

Materials

• Item 1
• Item 2
• Stock Solution 1
• Stock Solution 2

Stock Solutions

Stock Solution 1

• This is a very simple solution, so we only need a one line description of how to make it.

Stock Solution 2

This is a more involved solution, so we will describe how to make it in several steps:

1. Step 1
2. Step 2
3. Step 3

Protocol

1. Step 1
2. Step 2
3. Step 3

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

1. List troubleshooting tips here.
2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding ’’‘*~~~~’’’: to the beginning of your tip.

References

Gietz, R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS CARRIER DNA/PEG METHOD. Methods in Enzymology 350: 87-96.

Contact

• Bin He 22:01, 21 Sep 2018 (EDT)